| AdventHealth University | Orlando, FL | Regional | 3.20 |
| Augusta University | Augusta, GA | Regional | 3.00 |
| Barry University | Hollywood, FL | Regional | 3.20 |
| Endeavor Health | Evanston, IL | Regional | 3.50 |
| Fairfield University | Fairfield, CT | Regional | 3.20 |
| Florida Gulf Coast University | Fort Myers, FL | Either | 3.00 |
| Florida State University | Panama City, FL | Regional | 3.00 |
| George Fox University | Newberg, OR | Regional | 3.00 |
| California State University Fullerton | Pasadena, CA | Regional | 3.20 |
| Keiser University | Naples, FL | Regional | |
| Loma Linda University | Loma Linda, CA | Regional | 3.00 |
| Louisiana State University Health Sciences Center | New Orleans, LA | Either | 3.00 |
| Medical University of South Carolina | Charleston, SC | Regional | 3.00 |
| Middle Tennessee School of Anesthesia | Madison, TN | Regional | 3.00 |
| Midwestern University | Glendale, AZ | Regional | 3.00 |
| Metropolitan State University | Saint Paul, MN | Regional | 3.00 |
| New Mexico State University | Las Cruces, NM | Regional | 3.00 |
| Ohio University | Athens, OH | Regional | 3.20 |
| Old Dominion University | Virginia Beach, VA | Regional | 3.00 |
| Oregon Health and Science University | Portland, OR | Regional | 3.00 |
| Professional University Dr. Carlos J. Borrero Rios | Hato Rey, PR | Either | 3.00 |
| Gonzaga University | Spokane, WA | Regional | 3.00 |
| Rhode Island College | Providence, RI | Regional | 3.00 |
| Roseman University of Health Sciences | Henderson, NV | Regional | 3.20 |
| Rush University | Chicago, IL | Regional | 3.00 |
| Saint Mary's University of Minnesota | Minneapolis, MN | Regional | 2.75 |
| Samuel Merritt University | Oakland, CA | Regional | 3.00 |
| Texas Christian University | Fort Worth, TX | Either | |
| The University of Alabama at Birmingham | Birmingham, AL | Regional | 3.00 |
| The University of Arizona | Tucson, AZ | Regional | 3.00 |
| The University of Tennessee Health Science Center | Memphis, TN | Regional | 3.00 |
| Thomas Jefferson University | Philadelphia, PA | Regional | 3.20 |
| University of Cincinnati | Cincinnati, OH | Regional | 3.40 |
| University of Evansville | Evansville, IN | Regional | 3.00 |
| University of Kansas | Kansas City, KS | Either | 3.00 |
| University of Louisville | Louisville, KY | Regional | 3.00 |
| University of Miami | Coral Gables, FL | Regional | 3.00 |
| University of Michigan-Flint | Flint, MI | Regional | 3.00 |
| University of Mobile | Mobile, AL | Regional | 3.00 |
| University of Nevada Las Vegas | Las Vegas, NV | Regional | 2.75 |
| University of New England | Portland, ME | Regional | 3.00 |
| University of North Florida | Jacksonville, FL | Regional | 3.00 |
| University of Southern California | Los Angeles, CA | Regional | 3.00 |
| University of Tennessee at Chattanooga | Chattanooga, TN | Either | 3.00 |
| University of Wisconsin-Oshkosh | Oshkosh, WI | Regional | 3.00 |
| University of the Incarnate Word | San Antonio, TX | Regional | 3.20 |
| Ursuline College | Pepper Pike, OH | Regional | 3.00 |
| Virginia Commonwealth University | Richmond, VA | Regional | 3.00 |
| Wake Forest University School of Medicine | Winston Salem, NC | Either | 3.00 |
| West Virginia University | Morgantown, WV | Both | 3.00 |
| Western Carolina University | Asheville, NC | Either | 3.00 |
| Westminster University | Salt Lake City, UT | Regional | 3.00 |
| York College of Pennsylvania | York, PA | Either | 3.00 |